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Short Biography

I am a Lecturer at Queen's University Belfast. I obtained my Docent (Habilitation) title at the Faculty of Medicine in Umeå University, Sweden. Previous to that, I gained international experience during two postodctoral stays. The first was at the Institut Pasteur in Paris thanks to an EMBO fellowship, and the second postdoctoral stay was at the Institute for Molecular Pathology in Vienna. My doctoral work was performed at the International Max Planck Research School & University of Technology Dresden in Germany. I obtained my university education (Licenciatura) at the Universidad Nacional Autónoma de México (UNAM) in Mexico City.

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We have moved! The Cisneros lab is now located at Queen's University Belfast in Northern Ireland, UK. We are are up and running here. 


Publications : 28

Citations: 2005 / 3028




Sharma A., Aden R.O., Puhar A., Cisneros D.A. (2023). CRISPR-Cas guided mutagenesis of chromosome and virulence plasmid in Shigella flexneri by cytosine base editing. mSystems. 8.1:e01045-22. Open access link

Tadala L., Langenbach D., Dannborg M., Cervantes-Rivera R., Sharma A., Vieth K., Rieckmann L., Wanders M., Cisneros D.A., Puhar A., (2022). Infection-induced membrane ruffling initiates danger and immune signaling via the mechanosensor PIEZO1. Cell Reports. 40:111173 Open acces link

Graffeuil, A., Guerrero‑Castro, J., Assefa A., Uhlin, B.E. and Cisneros DA (2022). Polar mutagenesis of polycistronic bacterial transcriptional units using Cas12a. Microbial Cell Factories, 21:139. Open access  link

Hagberg A, Rzhepishevska O, Semenets A, Cisneros DA, Ramstedt M (2020). Surface analysis of bacterial systems using cryo‐X‐ray photoelectron spectroscopy. Surface and Interface Analysis, 52(12):792-801

Morales C., Ruiz-Torres M., Rodriguez-Acebes S., Lafarga V., Rodríguez-Corsino M., Megías D., Cisneros D.A., Peters J.M., Méndez J., Losada A (2020). PDS5 proteins are required for proper cohesin dynamics and participate in replication fork protection. Journal of Biological Chemistry, 295(1):146-57.

Muhar, M., Ebert, A., Neumann, T., Umkehrer, C., Jude, J., Wieshofer, C., Rescheneder, P., Lipp, J.J., Herzog, V.A., Reichholf, B., Cisneros, D.A. Hoffmann, T., Schlapansky, M.F. Bhat, P., von Haeseler, A., Köcher, T., Obenauf, A.C., Johannes Popow, J., Ameres, S.L., Zuber J., (2018) SLAM-seq defines direct gene-regulatory functions of the BRD4-MYC axis. Science , 360(6390), 800-805.


*Wutz G., *Várnai C., *Nagasaka K. , *Cisneros D.A., Stocsits R.R., Tang W., Schoenfelder S.,

Jessberger G., Muhar M., Hossain M.J., Walther N., Koch B., Kueblbeck M., Ellenberg J., Zuber J., Fraser P., Peters J.M (2017) . Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins. EMBO J. 36(24), 3573-3599.


  • Comment in: Haarhuis J.H.I. & Rowland B.D. (2017) ‘Cohesin: building loops, but not compartments’ EMBO J. 36(24), pp. 3549-3551


Ladurner, R., Kreidl, E., Ivanov, M. P., Ekker, H., Idarraga-Amado, M. H., Busslinger, G. A., Wutz, G., Cisneros, D. A. and Peters, J. M. (2016) 'Sororin actively maintains sister chromatid cohesion', EMBO J. , 35(6), pp. 635-653.


Davidson, I. F., Goetz, D., Zaczek, M. P., Molodtsov, M. I., in't Veld, P. J. H., Weissmann, F., Litos, G., Cisneros, D. A. , Ocampo-Hafalla, M., Ladurner, R., Uhlmann, F., Vaziri, A. and Peters, J. M. (2016) 'Rapid movement and transcriptional re-localization of human cohesin on DNA', EMBO J. , 35(24), pp. 2671-2685.


Amorim, G. C., Cisneros, D. A. , Delepierre, M., Francetic, O. and Izadi-Pruneyre, N. (2014) 'H-1, N-15 and C-13 resonance assignments of PpdD, a type IV pilin from enterohemorrhagic Escherichia coli ', Biomolecular NMR Assignments , 8(1), pp. 43-46.


Tedeschi, A., Wutz, G., Huet, S., Jaritz, M., Wuensche, A., Schirghuber, E., Davidson, I. F., Tang, W., Cisneros, D. A. , Bhaskara, V., Nishiyama, T., Vaziri, A., Wutz, A., Ellenberg, J. and Peters, J. M. (2013) 'Wapl is an essential regulator of chromatin structure and chromosome segregation', Nature , 501(7468), pp. 564-568.


Campos, M., Cisneros, D. A. , Nivaskumar, M. and Francetic, O. (2013) 'The type II secretion system - a dynamic fiber assembly nanomachine', Research in Microbiology , 164(6), pp. 545-555.


Cisneros, D. A. , Pehau-Arnaudet, G. and Francetic, O. (2012) 'Heterologous assembly of type IV pili by a type II secretion system reveals the role of minor pilins in assembly initiation', Molecular Microbiology , 86(4), pp. 805-818.


  • Comment in: Burrows, L. L. (2012). Prime time for minor subunits of the type II secretion and type IV pilus systems. Molecular microbiology , 86 (4), pp. 765-769.


Cisneros, D. A. , Bond, P. J., Pugsley, A. P., Campos, M. and Francetic, O. (2012a) 'Minor

pseudopilin self-assembly primes type II secretion pseudopilus elongation', EMBO J. , 31(4), pp.



Campos, M., Nilges, M., Cisneros, D. A. and Francetic, O. (2010) 'Detailed structural and assembly model of the type II secretion pilus from sparse data', PNAS , 107(29), pp. 13081-13086.


Tellez, L. A., Blancas-Mejia, L. M., Carrillo-Nava, E., Mendoza-Hernandez, G., Cisneros, D. A. and Fernandez-Velasco, D. A. (2008) 'Thermal Unfolding of Triosephosphate Isomerase from Entamoeba histolytica : Dimer Dissociation Leads to Extensive Unfolding', Biochemistry , 47(44), pp. 11665-11673.


Cisneros, D. A. , Oberbarnscheidt, L., Pannier, A., Klare, J. P., Helenius, J., Engelhard, M., Oesterhelt, F. and Muller, D. J. (2008) 'Transducer binding establishes localized interactions to tune sensory rhodopsin II', S tructure , 16(8), pp. 1206-1213.


  • Comment in: Hoff, W. D., & Spudich, J. L. (2008) . Single-Molecule Tour de Force: Teasing Apart a Signaling Complex. Structure , 16 (8), pp 1149-1150.


Taubenberger, A., Cisneros, D. A. , Friedrichs, J., Puech, P. H., Muller, D. J. and Franz, C. M. (2007) 'Revealing early steps of alpha(2)beta(1) integrin-mediated adhesion to collagen type I by using single-cell force spectroscopy', Molecular Biology of the Cell , 18(5), pp. 1634-1644.


Shah, D. S., Thomas, M. B., Phillips, S., Cisneros, D. A. , Le Brun, A. P., Holt, S. A. and Lakey, J. H. (2007) 'Self-assembling layers created by membrane proteins on gold', Biochemical Society

Transactions , 35, pp. 522-526.

Preiner, J., Janovjak, H., Rankl, C., Knaus, H., Cisneros, D. A. , Kedrov, A., Kienberger, F., Muller, D. J. and Hinterdorfer, P. (2007) 'Free energy of membrane protein unfolding derived from single-molecule force measurements', Biophysical Journal , 93(3), pp. 930-937.


Cisneros, D. A. , Friedrichs, J., Taubenberger, A., Franz, C. M. and Muller, D. J. (2007) 'Creating

ultrathin nanoscopic collagen matrices for biological and biotechnological applications', Small, 3(6), pp. 956-963.


Janovjak, H., Kedrov, A., Cisneros, D. A. , Sapra, K. T., Struckmeier, J. and Muller, D. J. (2006)

'Imaging and detecting molecular interactions of single transmembrane proteins', Neurobiology of Aging , 27(4), pp. 546-561.


Cisneros, D. A. , Muller, D. J., Daud, S. M. and Lakey, J. H. (2006) 'An approach to prepare membrane proteins for single-molecule imaging', Angewandte Chemie-International Edition , 45(20), pp. 3252-3256.


Cisneros, D. A. , Hung, C., Franz, C. A. and Muller, D. J. (2006) 'Observing growth steps of collagen self-assembly by time-lapse high-resolution atomic force microscopy', Journal of Structural Biology , 154(3), pp. 232-245.


Poole, K., Khairy, K., Friedrichs, J., Franz, C., Cisneros, D. A. , Howard, J. and Mueller, D. J. (2005) 'Molecular-scale topographic cues induce the orientation and directional movement of fibroblasts on two-dimensional collagen surfaces', Journal of Molecular Biology , 349(2), pp. 380-386.

Cisneros, D. A. , Oesterhelt, D. and Muller, D. J. (2005) 'Probing origins of molecular interactions stabilizing the membrane proteins halorhodopsin and bacteriorhodopsin', Structure , 13(2), pp.



  • Comment in: Chin, G. ‘Editor’s Choice: Deconstructing Membrane proteins’, Science , 307(5713), p. 1171.


*Cisneros, D. A., *Montero-Moran, G. M., Lara-Gonzalez, S. and Calcagno, M. L. (2004) 'Inversion of the allosteric response of Escherichia coli glucosamine-6-P deaminase to N-acetylglucosamine 6-P, by single amino acid replacements', Archives of Biochemistry and Biophysics , 421(1), pp. 77-84.



Pérez- Armendariz, Lamoyi, E., Mason, J. I., Cisneros-Armas, D. , Luu- The, V., & Bravo Moreno, J. (2004) ‘Developmental regulation of connexin 43 expression in fetal mouse testicular cells’, The Anatomical Record , 264 (3), pp. 237-246.

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